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KMID : 1134820120410101371
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 10 p.1371 ~ p.1377
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Antioxidant and Inhibitory Effects of Korean Panax ginseng Extract on Pro-inflammatory Mediators in LPS-stimulated RAW264.7 Macrophages
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Kim Ye-Jin
Son Dae-Yeul
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Abstract
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Biological activities of Korean Panax ginseng 55% ethanol extract (KPGE) were investigated. The measured total polyphenol content of KPGE was 357.45 mg/100 g. KPGE showed the highest α,α-diphenyl-β-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) radical scavenging activities of 80% and 86% at 1,000 μg/mL, respectively. DPPH and ABTS radical scavenging activities significantly increased in a KPGE concentration-dependent manner. SOD-like activity of KPGE (1, 10, and 100 μg/mL) increased from 22% up to 33% at KPGE concentrations of 500 and 1,000 μg/mL. KPGE treatment significantly suppressed the generation of pro-inflammatory mediators, including nitric oxide (NO), prostaglandin E2 (PGE2), and cytokines (tumor necrosis factor-alpha: TNF-α, interleukin-6: IL-6, interleukin-1β: IL-1β), in lipopolysaccharide (LPS)- stimulated RAW 264.7 macrophages. KPGE demonstrated strong anti-inflammatory activity that reduced NO and PGE2 production in LPS-stimulated RAW 264.7 cells. Even low concentrations of KPGE (1 and 10 μg/mL) reduced PGE2 and NO production in RAW 264.7 macrophages without LPS-stimulation, respectively. At concentrations of 100, 500, and 1,000 μg/mL, TNF-α, IL-1β and IL-6 production were significantly suppressed. The results of our study suggest the potential of Korean Panax ginseng as an excellent antioxidant substance for inhibiting inflammatory mediators. Therefore, Korean Panax ginseng (KPGE) may be used as a therapeutic approach to various inflammatory diseases.
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KEYWORD
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antioxidant, Korean Panax ginseng, nitric oxide, polyphenol, pro-inflammatory
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